肺癌细胞株中层粘连蛋白5多态性与其表达水平的关系

摘要

Objective To explore the relationships between the polymorphisms in the 5'-UTR region of laminin 5 (LN5) and the expression level in lung cancer cell lines. Methods The polymorphisms of LN5 and the mRNA expression levels in 12 lung cancer cell lines were detected, using sequencing and real-time PCR methods, respectively, the relationships between them were analyzed. Results Cell lines with GA genotype of -183G/A polymorphisms have a higher mRNA expression level of LN5 than the cell lines with GC genotype (t= 7.136,P<0.001). No relationships of -6C/A and -89A/G polymorphisms and the mRNA expression levels were found in this study. Conclusions This study suggestes that - 183G/A polymorphisms of LN5 gene maybe regulate the transcriptional level, and these results provide the basic evidence for the further mechanism study.%目的 探讨肺癌细胞株中层粘连蛋白5(LN5)5’非翻译区多态性与其表达水平的关系.方法 应用测序及实时定量PCR方法检测12株肺癌细胞株中LN5 5’非翻译区多态性和LN5基因mRNA表达水平,分析相互之间的关系.结果 LN5 5’非翻译区- 183G/A中GA基因型的LN5 mRNA表达水平明显高于GG基因型(t=7.136,P＜0.001).本研究中尚未发现LN5基因-6C/A和-89A/G多态性位点与其mRNA表达的相关性.结论 本研究提示LN5启动子区-183G/A多态性位点具有转录调控功能,为进一步的机制探讨提供了依据.