Arsenic(As)speciation for the phytoremediation by the Chinese brake fern was studied.In particular,the mechanism of how plants induce compounds containing thiol(SH)and proteins by As exposure in terms of the relationship between As and phosphate uptaken into plant cells was examined.Pteris vittata callus could effciently reduce As(V)to As(III)by the rapid introduction of reductase and synthesize thiols leading to phytochelatins production.Furthermore,Pteris vittata could control phosphate concentration in the cells corresponding to the concentration of arsenite and arsenate.To our best knowledge,this is the first report to show the mechanisms of such high As tolerance of Pteris vittata using their callus in terms of in vitro approach for the analysis of As speciation and metabolism route.
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