Perfluoroalkyl substances(PFASs) are a class of fluorine substituted carboxylic acid, sulfonic acid and alcohol, structurally similar to their corresponding parent compounds. Previous study demonstrated the potential endocrine disruption and reproductive toxicity of perfluorooctane sulfonic acid and perfluorooctanoic acid, two dominant PFASs in animals and humans. We explored the relationship between eleven perfluoroalkyl acids(PFAAs)with different carbon chain length and their ability to inhibit progesterone production in mouse Leydig tumor cells(m LTC-1). We found an obvious dose–response relationship between progesterone inhibition rate and PFAA exposure concentration in m LTC-1. The relative inhibition rate of progesterone by PFAAs was linearly related to the carbon chain length and molar refractivity of PFAAs. Mitochondrial membrane potential(MMP) decreased after PFAA exposure at the half-maximal inhibitory effect concentration(IC_(50)) of progesterone production in m LTC-1, while the reactive oxygen species(ROS) content increased significantly. These results imply that the inhibition effect of PFAAs on progesterone production might be due, in part, to ROS damage and the decrease in MMP in m LTC-1.
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机译:Possible Negative Regulatory Effects of Antibiotic Production in a Hetrologous System by Sare_4854, a Novel Member of Streptomyces Antibiotic Regulatory Protein Family (SARP) from a Marine Microorganism Salinispora arenicola CNH643 DSM 44819AU Shu, Xiao Zhang, Wei Yu, Jinlong Wu, Shaowen Ma, Yanling Zhu, Minzhe Xia, Sisi Xia, Shichao Zhang, Hao Li, Aiying (aiyli@126.com) Qi, Chao (qichao@mail.ccnu.edu.cn)