The impacts of landfill leachate irrigation on methane oxidation activities and methane-consuming bacteria populations were studied by incubation of landfill cover soils with leachate and(NH 4) 2 SO 4 solution at different ammonium concentrations.The community structures and abundances of methane-oxidizing bacteria(MOB) and ammonia-oxidizing bacteria(AOB) were examined by PCRDGGE and real-time PCR.Compared with the pure(NH 4) 2 SO 4 solution,leachate addition was found to have a positive effect on methane oxidation activity.In terms of the irrigation amount,ammonium in leachate was responsible for the actual inhibition of leachate.The extent of inhibitory effect mainly depended on its ammonium concentration.The suppression of the predominant methaneconsuming bacteria,type I MOB,was responsible for the decreased methane oxidation activity by ammonium inhibition.Methaneconsuming bacteria responded diversely in abundance to ammonium.The abundance of type I MOB decreased by fivefold;type II MOB showed stimulation response of fivefold magnification upon the first addition but lessened to be lower than the original level after the second addition;the amount of AOB was stimulated to increase for 20-30 times gradually.Accumulated nitrate from nitrification strengthened the ammonium inhibition on type I and type II MOB,as a result,repetitive irrigation was unfavorable for methane oxidation.
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State Key Laboratory of Pollution Control and Resources Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China;
