威灵仙总皂苷诱导急性早幼粒细胞白血病细胞株NB4分化作用的研究

摘要

Objective:To study the effect of total saponin from clematis chinensis osbeck on differentiation of a-cute promyelocytic leukemia NB4 cell line cultured in vitro. Methods:The NB4 cells were independently treated with ATRA and total saponin combined with differemt concentrations in 5 days.The proliferative inhibition rates of NB4 cell induced by total saponin and ATRA were calculated in every day. Cell differentiations were demonstrated by Giemsa stain, transmission electron microscope (TEM) and NBT reduction assay at different time. The expressions of CD1lb and CD33 induced by these drugs were measured by flow cytometry (FCM) at different times. Results:As compared with blank control group,that the NB4 cells were inhibited significantly by the clematis total saponins showed a dose-effect dependence. There were no statistical significances in the differentiation phe-nomenons. Conclusion:The total saponin can obviously inhibit the proliferation of NB4 cells. However, there were no obvious differentiation effect in the NB4 cells treated by total saponin from clematis chinensis osbeck .%目的 研究威灵仙总皂苷对NB4细胞分化的影响.方法 取对数生长期的NB4细胞,设置多个浓度梯度的威灵仙总皂苷药物组,观察用药后不同时间点NB4活细胞数,计算生长抑制率;Wright's-Giemsa染色计数法和NBT还原试验测定细胞分化率,透射电镜观察细胞形态学变化;流式细胞术检测CD11b/CD33表达情况.结果 与空白对照组比较,威灵仙总皂苷对NB4细胞有明显的生长抑制作用,且具有剂量-效应依赖关系;但具有统计学意义的诱导分化现象不明显.结论 威灵仙总皂苷可有效抑制NB4细胞增殖,但对NB4细胞无明显的诱导分化作用.