淫羊藿总黄酮对心力衰竭大鼠心肌细胞凋亡的调控作用研究

摘要

ObjectiverTo investigate the anti-apoptotic effects of total flavonoids of epimedium(TFE) on isopro-terenol-induced congestive heart failure (CHF) in rats. Methods:CHF was induced in male Sprague-Dawley rats by receiving subcutaneous injection of isoproterenol. Rats with heart failure were randomly divided into the control group,the metoprolol group,the TFE-low-dose group,the TFE-middle-dose group and the TFE-high-dose group. Meanwhile,rats receiving injection of saline serve were the normal group. Some indices were observed in these groups including the left ventricular weight index(LVWI) ,the hemodynamic changes,the neuroendocrine parameters changes and the myocardial apoptosis indices. Results:Compared with the control group,TFE dose-depen-dently reversed the changes of LVWI and hemodynamic parameters. In addition,TFE significantly inhibited serum levels of TNF-a,NE,AngⅡ in rats with CHF. DNA fragments were obviously observed in the control group. The protein expressions of Bax, Caspase-9 and Caspase-3 were decreased and Bcl-2 was increased significantly in the TFE group as compared with those of the control group. Conclusion:TFE as well as metoprolol can decrease myocardial cell apoptosis and ameliorates LV dysfunction in isoproterenol-induced CHF in Rats.%目的 研究淫羊藿总黄酮对异丙肾上腺素(ISO)所致充血性心力衰竭大鼠心肌细胞凋亡的影响.方法 取SD雄性大鼠,采用皮下注射ISO方法建立心力衰竭模型.将造模成功的动物随机分为模型组、美托洛尔组及淫羊藿总黄酮小、中、大剂量组,另取皮下注射生理盐水的正常大鼠作为正常组.观察各组动物左室质量指数,血流动力学变化,神经内分泌因子的变化及心肌细胞凋亡相关指标.结果 与模型组比较,淫羊藿总黄酮可呈剂量依赖性逆转左心室质量指数和血流动力学参数,明显抑制心力衰竭大鼠血清肿瘤坏死因子-α、去甲肾上腺素、血管紧张素Ⅱ水平,减轻心肌细胞DNA片断化,减少Bax、Caspse-9和Caspase-3表达,增加Bcl-2表达.结论 淫羊藿总黄酮与β受体阻滞剂美托洛尔均能抑制皮下注射ISO所致心力衰竭大鼠心肌细胞凋亡,改善心功能.