土贝母皂苷乙诱导非小细胞肺癌A549细胞凋亡的研究

摘要

目的 观察土贝母皂苷乙对非小细胞肺癌A549细胞增殖的抑制作用并探讨诱导其凋亡的机制.方法 用台盼蓝染色法检测细胞的活力;MTT法检测土贝母皂苷乙对细胞的抑制率;Hoechst33342/PI荧光双染法观察细胞的形态;用流式细胞仪检测土贝母皂苷乙对细胞凋亡率的影响.结果 土贝母皂苷乙能显著抑制A549细胞的增殖,24h的IC50为13.37 μg/mL,48 h的IC50为10.99μg/mL,并且呈时间和剂量依赖性.Hoechst33342/PI荧光双染法观察到经土贝母皂苷乙处理的细胞核皱缩、染色体聚集,核破裂形成小体,呈现明显的凋亡现象.FCM分析结果显示,随着药物浓度的增高,细胞的凋亡率依次升高,从4.56％增加至17.62％.该研究表明土贝母皂苷乙能显著促进A549细胞凋亡,且在一定范围内呈现剂量依赖效应.结论 土贝母皂苷乙能诱导非小细胞肺癌A549细胞凋亡.%Objective:To investigate the inhibitory effect of tubeimosides Ⅱ on the proliferation of non-small cell lung cancer A549 cells and the mechanism of its induction.Methods:Trypan blue staining method was used to detect cell viability;MTT was used to detect the inhibition rate detection of tubeimosides Ⅱ;Hoechst33342/PI double fluorescent staining method was used to to observe cell morphology;the effect of tubeimosides Ⅱ on cell apoptosis rate was detected with flow cytometry.Results:Tubeimosides Ⅱ could significantly inhibit the proliferation of A549 cells.IC50 of 24 h was 13.37 μg/mL,and IC50 of 48h was 10.99 μg/mL in a time and dose dependent manner.Hoechst33342/PI fluorescence double staining method was used to observe the nuclear shrinkage,chromosome aggregation,nuclear fragmentation,and apoptosis of the treated cells.FCM analysis showed.that with the increase of drug concentration,the apoptotic rate of cells increased sequentially,from 4.56％ to 17.62％.This study showed that tubeimosides Ⅱ could significantly promote the apoptosis of A549 cells,and showed dose-dependent effects in a certain range.Conclusion:Tubeimosides Ⅱ can induce apoptosis of non-small cell lung cancer A549 cells.