纳米β-硅酸钙/硒的制备及其抗菌性能

摘要

为进一步提高β-硅酸钙(β-CaSiO3)的抗菌活性,在通过油水界面法合成纳米β-CaSiO.的基础上,结合氧化还原法引入红色纳米硒(Se),制备出纳米β-硅酸钙/硒(β-CaSiO3/Se)复合物.采用X射线电子能谱(EDS)和X射线衍射(XRD)对β-CaSiO3/Se的成分和物相进行表征,进一步采用透射电子显微镜(TEM)观察样品微观形貌.选用大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)测试纳米β-CaSiO.和β-CaSiO3/Se的抗菌性能.结果表明,本方法成功制得纳米β-CaSiO3/Se复合物,其中纳米Se晶型为六方晶型.体外抗菌测试表明,纳米β-CaSiO3/Se对E.coli和S.aureus均具有优良的抗菌性能.纳米β-CaSiO3/Se与E.coli和S.aureus接触培养24 h的抑菌率分别达到(92±4)％和(95±4)％.纳米β-CaSiO3/Se有望作为新型多功效生物材料在生物医学领域获得应用.%Aimed to improve the antimicrobial activity of β-CaSiO3,nano-β-CaSiO3/Se compound was prepared by introducing red nano-Se with oxidation-reduction method,based on the synthesis of nano-β-CaSiO3 with oil-water interface method.The composition and phase of the nano-β-CaSiO3/Se were characterized by X-ray photoelectron spectroscopy (EDS) and X-ray diffraction (XRD),and the morphology of samples was observed by transmission electron microscopy (TEM).Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) were used to test the antibacterial property of nano-β-CaSiO3 and nano-β-CaSiO3/Se.Experimental results show that nano-Se is successfully supported on nanoβ-CaSiO3 with the crystal shape of hexagonal lattice.In addition,nano-β-CaSiO3/Se has excellent antibacterial properties to E.coli and S.aureus with the antibacterial rates of (92±4)％ and (95±4)％,respectively.Nano-β-CaSiO3/Se is expected to bea new multi-functional biomaterial in the field of biomedical applications.