Fabrication of PEI-Liposome Loaded Fish Gelatin Nanofibers Mats by Green Electrospinning

摘要

Polyethyleneimine( PEI) and cationic liposomes were widely used for gene delivery and the combination of PEI and liposome was reported to result in a higher efficiency of cell transfection in vitro.In recent years, better transfection was observed for the drug-loaded liposome fixed on the tissue engineering scaffolds via embedding,surface adsorption or covalent grafting thus protected and bound by the scaffolds.In the present study,a novel PEI-liposome loaded fish gelatin composite nanofiber was successfully fabricated by a green electrospinning process.The existence of PEIliposome in the composite nanofibers was determined by Fourier transform infrared( FTIR) spectra, transmission electron microscopy( TEM), and confocal laser scanning microscopy( CLSM).As shown by scanning electron microscopy( SEM),the electrospun composite nanofibers with uniform diameter were smooth and round,and the morphology of the fish gelatin fibers did not change significantly after the incorporation of PEI-liposomes.The transfection results in vitro suggest PEI-liposome loaded fish gelatin material may have a promising application in non-viral gene delivery systems.