Controllable Fabrication of Spider-Web-Like Structured Anaphe Panda Regenerated Silk Nanofibers / Nets via Electrospinning / Netting

摘要

An Anaphe panda silk nanofibers / nets( NFN) membrane with attractive structures consisting of common electrospun nanofibers and two-dimensional( 2D) spider-web-like structured nano-nets were successfully fabricated via electrospinning / netting technology. The unique structures of NFN membranes such as extremely small diameter( < 20 nm),high porosity,large specific surface area. and biocompatibility make this Anaphe panda silk NFN membrane a promising candidate for biomedical applications. In the present study, field emission scanning electron microscopy( FESEM) was used to investigate the influence of polymer solution and humidity on nano-nets coverage and morphology. The FE-SEM images revealed that nano-net coverage area increased with increase in concentration of solution while lowering the humidity increased the nano-nets formation. Moreover,the mechanical properties of the membrane were also tested and the result showed that the silk NFN membrane displayed a breaking stress of 3. 7 MPa and breaking strain of 13. 8%. For further structural elucidation, Fourier transform infrared spectroscopy( FT-IR) was used to analyze the structural conformation changes from random coil to β-sheet in the NFN membrane which was an important factor effecting the usability of membrane. Thus,the results above confirmed the feasibility of Anaphe panda NFN structures applicability in cell tissue culture and other biomedical applications.