Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

摘要

A new cross-linked heptapeptide matrix (CHM) was prepared and the adsorption performance for papain was investigated.Firstly,a phage-displayed heptapeptide (Ⅱe-Gln-Ser-Pro-His-Phe-Phe) with high affinity to papain was found.Secondly,the heptapeptide was cross-linked to a matrix for papain adsorption.Finally,the adsorption conditions were optimized by response surface methodology.The results indicated that the optimum adsorption conditions were determined as initial pH 6.9,temperature 40℃2,and the adsorption capacity of CHM for papain was found to be 55.52 mg/g.Moreover,the papain was purified 78-fold in a single step determined by affinity precipitation.More than 87 ％ of the adsorbed papain was desorbed using the eluent solventtriethylamine at pH 7.4.The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.