β-defensin has a broad-spectrum microbicidal activity and is an important regulator of innate immunity.The aim of this study was to examine the express of β-defensin l(Dcfbl) after forced weaning and natural weaning.72 female mice were divided into forced weaning group (n=6) and natural weaning group (n = 6) after parturition.At the day of weaning (0 d) and within 5 days after weaning (1 d, 2 d, 3 d, 4 d and 5 d) , the mice were euthanatized.The forth mammary gland tissue were taken and examined with semi-quantitative RT-PCR.The results showed that the expression of Defbl mRNA significantly decreased (P展开▼