Objectives To investigate the effect of 1,25-(OH)2D3 supplementation on airway remodeling in early life of asthmatic rat model, and the relationship between TGF-β1 expression and airway remodeling. Methods Thirty two mature female Wistar rats were randomly divided into four groups. From the seventh day of gestation, the rats in each group were given different doses of 1,25-(OH)2D- every other day by intragastric administration with 2 fig/kg, 10 jig/kg and 20 μg/kg for low, medium and high-dose group respectively. The control group was given with normal saline, until the newborn rats were 21 days old and wearied. Then the filial generation of rats was induced into asthma model. After sacrificed, hematoxylin and eosin (HE) staining was carried out to lung tissue to observe airway inflammation. And collagen staining with sirius red was observed by polarimicroscop, and the expression and localization of TGF-β1 in bronchi were measured. Results Compared with the control group, mild inflammation was observed in the medium dose group and serious inflammation was observed in high dose group. For smooth muscle thickness, no significant differences were found between the control group and the low and high dose groups (P＞0.05), while significant decrease was found in the middle dose group (P＜0.05). The thickness of collagen fibers and IOD in the control group did not show significant differences from both the high dose and the low dose groups (P＞0.05), but significant decrease was seen in the middle dose group (P＜0.05). Comparing to the control group, the expression of TGF-βl significantly decreased in the low dose group and the middle dose groups (P＜0.05), and significantly increased in the high dose group (P＜0.05). Conclusions Different doses of 1,25-(OH)2D3 supplementation can influence the airway remodeling in asthmatic rats.%目的 探讨不同剂量1,25-(OH)2D3干预对生命早期大鼠哮喘模型气道重塑的影响,以及TGF-β1表达调控与气道重塑的关系.方法 选用雌性Wistar大鼠32只,随机分为4组.受孕第7天起,以隔天灌胃的方式给予1,25-(OH)2D3,低剂量组 2 μg/kg,中剂量组 10 μg/kg,高剂量组 20 μg/kg,对照组以生理盐水代替,直到子代大鼠生后第21天离乳为止.其子代鼠制备成哮喘模型.子代鼠处死后肺组织行HE染色观察炎症反应;天狼猩红染色观察胶原染色分布;以及TGF-β1的表达分布.结果 与对照组相比,中剂量组炎症反应最轻而高剂量组较严重;对照组的平滑肌厚度与低、高剂量组相比差异无统计学意义 (P >0.05),与中剂量组相比差异有统计学意义 (P <0.05).对照组的光密度值,胶原纤维厚度与高、低剂量组相比差异无统计学意义 (P >0.05),与中剂量组相比差异有统计学意义 (P <0.05).免疫组织化学结果显示,与对照组相比,低、中剂量组TGF-β1的表达减低,高剂量组则表达明显增多,差异均有统计学意义 (P <0.05).结论 不同剂量1,25-(OH)2D3可以在一定程度上影响大鼠哮喘模型的气道重塑.
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