目的 本研究旨在研究神经母细胞瘤Wnt-5a基因的表达,并探讨其与肿瘤的临床分期、病理分型及对化疗的反应等的关系.方法 选择2003年至2008年住院治疗的77例神经母细胞瘤病例进行研究,其中恶性神经母细胞瘤病例(43例)和节细胞性神经母细胞瘤病例(19例)为实验组,良性神经节细胞瘤病例(15例)为对照组.采用蛋白质免疫印迹杂交(Western blotting)方法检测肿瘤组织中Wnt-5a基因的表达.结果 与对照组[光密度值(4.79±0.75)×105]相比,Wnt-5a基因在实验组中的表达[1、2、3、4和4 s期的光密度值分别为(2.87±0.43)×105、(2.13±0.35)×105、(2.07±0.44)×105、(1.73±0.11)×105]明显下调(P＜0.01).在实验组中,Wnt-5a基因在3、4和4 s期的表达[光密度值分别为(2.13±0.35)×105、(2.07±0.44)×105、(1.73±0.11)×105]显著低于1、2期[光密度值为(2.87±0.43)×105,P＜0.01)];4期病例中,Wnt-5a基因在神经母细胞瘤中的表达低于节细胞性神经母细胞瘤(P＜0.05).术前化疗的3、4期病例Wnt-5a基因的表达[光密度值分别为(2.13±0.35)×105、(2.07±0.44)×105]显著低于良性对照组[光密度值为(4.79±0.75)×105,P＜0.01)],也低于未予化疗的1、2期病例[光密度值为(2.87±0.44)×105,P＜0.01)].结论 Wnt-5a基因的表达与肿瘤的临床分期和恶性侵袭程度呈负相关.%Objective Wnt-5a gene is a member of the Wnt gene family. Wnt genes play an important role in embryogenesis and oncogenesis. The study aims to detect the expression of Wnt-5a gene at protein level in neuroblastoma, and further investigate the potential association of Wnt-5a gene with clinical stage, pathology classification and chemotherapy. Methods 77 neuroblastoma cases were collected for the research. The neuroblastoma cases(n=43) and ganglioneuroblastoma cases (n = 19) were divided into the experimental group and the ganglioneuroma cases(n = 15) were divided into the control group. The Western blotting method was used to detect the expression of Wnt-Sa gene at protein level in tissue samples. We analyzed the results combined with clinical documents and pathologic documents. Results The expression of Wnt-5a gene significantly reduced in the experimental group [stage 1, stage 2, stage 3, stage 4 and stage 4s were (2.87 ± 0.43) × 105 , (2.13 ± 0.35) × 105,(2.07 ± 0.44) × 105 and (1.73 ± 0. 11) × 103 respectively when compared with the control group[ (4.79 ± 0.75 ) × 105 ] (P <0.01). The expression of Wnt-5a gene in stage 3, stage 4 and stage 4s[(2.13 ± 0.35) × l05 , (2.07 ± 0.44) × 103 and (1.73 ± 0.11) × 105 respectively] were lower than stage 1 and stage 2[ (2.87 ± 0.43) × 105] (P <0.01). The expression of Wnt-5a gene in neuroblasltoma was lower than in ganglioneuroblastoma in stage 4(P<0.05). Although the cases completed courses of chemotherapy before surgery, the expression of Wnt-5a gene in stage 3 and stage 4[ (2. 13 ± 0.35) × 105 and (2.07 ± 0.44) × 105 respectively]were lower than control group [ (4. 79 ±0. 75) × 105 ] and stage 1 and stage 2[(2. 87 ± 0.44) × 105 ] which were not administrated chemotherapy(P < 0. 01). Conclusion The expression of Wnt-5a gene presents the negative correlation with clinical stage and the tumor's malignancy and invasion.
展开▼
