上皮细胞钙粘蛋白与β-连环蛋白在恶性腹水鉴别诊断中的价值

摘要

Objective Analysis the levels of E-cadherin and β-catenin in neoplastic,hepatic cirrhotic and tuberculous ascitic fluid to determine the value of E-cadherin and β-catenin for differentiating[min1]. Methods E-cadherin andβ-catenin levels were detected in ascitic fluid from 5 patients with tuberculous disease,11 patients with cirrhosis,and 25 patients with neoplasm by EL ISA methods. Results The level of E-cadherin was 7.57±0.48 ng/ L in tuberculous group,8.18±0.81 ng/ L in cirrhosis group,and 9.35±1.84 ng/ L in neoplastic group.The level of β-catenin was 0.76±0.13 ng/ L in tuberculous group,0.93±0.06ng/ L in cirrhotic group,and 1.67±1.16 ng/ L in neoplastic ascites .E-cadherin andβ-catenin in patients with neoplasm were much higher than those with tuberculous disease and cirrhosis(P＜0.05).No difference of those between hepatic cirrhosis and tuberculous peritonitis was found.There was positive correlation between the expression of E-cadherin andβ-catenin(P＜0.01,r=0.479).Conclusion E-cadherin and β-catenin expression is involved in the incursion and metastasis of the neoplasm,and the levers of them were significantly elevated in neoplastic group.So there would be some difference between malignancy-related and no-malignancy-related ascites.The detection of E-cadherin andβ-catenin levels is useful in the differential diagnosis of benign and malignant ascites.%目的 通过检测不同病因腹水中上皮细胞钙粘蛋白(E-cadherin)和β-连环蛋白(β-catenin)的含量,探讨其在鉴别良、恶性腹水性质中的意义.方法 收集41例来自临床上已确诊患者的腹水标本,分为结核组(5例)、肝硬化组(11例) 和恶性肿瘤组(25例) 3 组,采用酶联免疫吸附法( ELISA)检测E-cadherin和β-catenin的含量.结果 结核性腹水E-cadherin水平为(7.57±0.48) ng/L,肝硬化腹水水平为(8.18±0.81) ng/L,恶性腹水水平为(9.35±1.84) ng/ L.结核性腹水β-catenin水平为(0.76±0.13) ng/L,肝硬化腹水为(0.93±0.06) ng/L,恶性腹水为(1.67±1.16) ng/L.恶性腹水E-cadherin和β-catenin水平明显高于结核性腹水和肝硬化性腹水(P＜0.05),两者水平在结核性腹水和肝硬化腹水之间比较差异无显著性(P>0.05).相关分析表明E-cadherin和β-catenin的表达具有正相关性(P＜0.01,r=0.479).结论 E-cadherin和β-catenin与肿瘤的浸润和转移有关,恶性腹水中两者的水平显著增高.E-cadherin和β-catenin对良、恶性腹水鉴别诊断有重要价值.