结直肠癌患者血清 miRNA-200b、miRNA-200c水平表达及其与临床特征的关系研究

摘要

目的：探讨结直肠癌患者血清中 miRNA（miR）-200b 和 miR-200c 的表达及其与患者临床病理特征及预后的关系。方法采用实时定量聚合酶联反应（PCR）方法检测90例结直肠癌患者（观察组）术前血清标本和50例健康志愿者（对照组）血液样品中 miR-200b 和 miR-200c 的表达水平，并结合患者临床特征进行分析。结果观察组血清 miR-200b 和 miR-200c 的表达水平明显低于对照组。miR-200b 和 miR-200c 的表达与肿瘤组织分化程度、TNM分期、淋巴结转移密切相关；miR-200b 或 miR-200c 低表达与不良预后有关（P ＜0．01）。结论结直肠癌患者血清中 miR-200b 和miR-200c 的表达与组织学分化、临床分期、淋巴结转移有关。血清 miR-200b 和 miR-200c 的检测有可能作为结直肠癌诊断及判断预后的生物标志。%Objective To explore the expression of serum miR-200b and miR-200c and the correlation with clinical features in colorectal cancer patients.Methods 90 colorectal cancer patients (observation group)and 50 healthy volunteers(control group)were detected to explore the expression of miR-200b and miR-200c by real-time quantitative polymerase chain reaction (qRT-PCR),and the relationship between miR-200b and miR-200c and clinical features was analyzed.Results The expression levels of serum miR-200b and miR-200c in observation group were significant lower than that in the control group.Relationships between expression of serum miR-200b and miR-200c and clinic features were observed in histological differentiation,TNMstage,lymph node metastasis.Lower expression of miR-200b and miR-200c had poorer prognosis in colorectal cancer patients(P <0.01).Conclusion The expression of serum miR-200b and miR-200c in patients with colorectal cancer had related to histo-logical differentiation,TNMstage,lymph node metastasis.The detection of serum miR-200b and miR-200c might be used as the biomarkers of early diagnosis and estimating prognosis of colorectal cancer.