ADMA引起肾小球内皮细胞内质网应激及槲皮素的抑制作用

摘要

Objective To study the effect of quercetin on ADMA induced endoplasmic reticulum stress response ( ER stress ). Methods Glomerular endothelial cells were cultured, and left untreated or challenged for 24 h with 100μM ADMA in absence or presence of 100 μM quercetin, and PERK, ATF4 and CHOP were determined by Western blotting. Results ADMA increased the level of PERK, ATF4 and CHOP, and quercetin inhibited the expression of PERK, ATF4 and CHOP induced by ADMA. Conclusion ADMA can induce ER stress while quercetin may inhibit ADMA - induced ER stress response.%目的 探讨槲皮素对非对称性二甲基精氨酸(ADM)A引起的内质网应激的影响.方法 对肾小球内皮细胞进行培养,细胞分为5组,阴性对照组,ADMA组,槲皮素组,ADMA+槲皮素组,阳性对照组,经过加入相应刺激后继续培养细胞24 h,通过Western blot方法检测内质网应激蛋白PERK、ATF4及CHOP的水平.结果 ADMA组较阴性对照组P-PERK、ATF4及CHOP的表达明显升高,而ADMA+槲皮素组较ADMA组P-PERK、ATF4及CHOP的表达水平明显下降.结论 ADMA可以引起肾小球内皮细胞内质网应激,而槲皮素可以抑制ADMA引起的内质网应激.