Objective To survey the distribution of Candida species and drug susceptibility testing of isolates from sterile areas and to pro-vide the basis for clinical anti - fungus therapy. Methods After cultivation and isolation,all strains were identified with chromogenic media or VITEK - 2 Compact 2. ATB FUNGUS3 kits were used for drug susceptibility testing. Results A total of 939 strains of Candida had been isolated from sterile areas of patients during January 2013 to December 2014. Candida albicans(49. 31% )was the most common pathogen,followed by Candida glabrata(28. 43% )and Candida tropicalis(12. 03% ). ICU and cadre health care ward were high - risk departments. All isolates were sensitive to amphotericin B(AMB)and flucytosine. Except Candida krusei was naturally fluconazole - resistant,Candida albicans was more sensi-tive to azoles than Candida glabrata and Candida tropicalis. Conclusion Candida albican is the main pathogen isolated from sterile areas on the whole,but Candida glabrata is the primary isolate in clean mid - urine specimens and less sensitive to azoles. Clinicians should pay more attention to sterile samples for laboratory examination and antifungal agents should be applied according to the results of drug susceptibility testing.%目的：分析临床无菌体液标本中分离的常见念珠菌的临床分布情况及其对临床常用抗真菌药物的敏感性，为临床抗真菌治疗提供依据。方法各种临床标本经分离培养后，CHROMagar 念珠菌显色培养基或 VITEK -2 Compact 2鉴定；ATB FUNGUS 3药敏试剂条测定常用抗真菌药物敏感性。结果收集2013年1月至2014年12月无菌部位分离念珠菌939株，主要为白色念珠菌（49.31％）、光滑念珠菌（28.43％）和热带念珠菌（12.03％）。高发科室主要为 ICU 和干部保健病房。临床分离的念珠菌对两性霉素 B 和5-氟胞嘧啶均敏感，除克柔念珠菌对氟康唑天然耐药外，光滑念珠菌和热带念珠菌对唑类药物敏感性相对较差，而白色念珠菌对唑类药物的敏感性较高。结论临床无菌部位感染以白色念珠菌为主，其中尿液标本分离的念珠菌以光滑念珠菌为主，且其对唑类药物敏感性下降，临床医生应重视无菌体液标本的送检，并根据药敏结果合理使用抗真菌药物。
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