Developing 41 new microsatellite markers in red tail prawns,Fenneropenaeus penicillatus

摘要

Fenneropenaeus penicillatus is an endangered species in China due to overfishing and damage to or loss of habitat. In this study, 41 new microsatellite markers were developed by using the FIASCO protocol with subsequent analysis on 30 wild individuals from Ningde, China. Our analysis shows that 35 loci were polymorphic and six loci were monomorphic. For the 35 polymorphic loci, the number of alleles ranged from 2 to 7, with an average of 3.6571. The polymorphism information content ranged from 0.121 to 0.794. The observed heterozygosity rate ranged from 0.0000 to 0.8889, and expected heterozygosity ranged from 0.1111 to 0.8129. However, 12 loci did not conform to Hardy-Weinberg equilibrium after Bonferroni correction. This is the first time the genetic diversity of F. penicillatus has been explored in Ningde. This group is at a low level of genetic diversity;therefore further measures should be taken to protect this species. Furthermore, these new microsatellite markers will be useful for the future conservation of F. penicillatus.