导致不良妊娠的多病原体因素检测分析及临床意义

摘要

Objective　To analysis the relationship between the multipathogens infection and unhealthy pregnancy.Methods　All patients was selectively divided into three groups:group A including 106 patients with unhealthy pregnancy,group B:55 gravidas with normal induced abortion,group C:55 normal parturient with term labor.The ten kinds of pathogens from abortion tissues and placentas were tested by PCR method respectively.Anti-pathogen IgM antibodies were detected by ELISA in the blood specimens.Results　The distribution status of the pathogens were as followed:Human parvovirus B19(B19V 23.6%)＞Cytomegalovirus(CMV,16.0%)＞Toxoplasma (TOX，15.1%)＞Rubella virus(RV，10.4%)＞Ureaplasma urealyticum(9.4%)＞Coxsackie(7.5%)＞Herpes simplex virus(HSV,6.6%)＞Adenovirus,Eprstan Barr virus(3.8%)＞Chlamydia trachomatic(CT 2.8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B19V,CMV,RV,HSV,TOX were significant difference(χ2 test,P＝0.000～P＝0.031) among the three groups,hovever others were not(χ2 test,P＝0.121～P＝0.724).Conclusions　The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B19V，CMV，TOX，RV，HSV.These pathogens infection should be detected timely in gestational period.%目的探讨B19V等10种病原体感染与人类不良妊娠之间的关系。方法采集静脉血液、流产组织和胎盘组织为标本，应用PCR方法检测组织标本各种病原体，应用ELISA检测血液标本相应的IgM的抗体。结果病原体在不良妊娠组中的分布呈B19Ⅴ(23.6%)＞CMV(16.0%)＞TOX(15.1%)＞RV(1064%)＞UU(9.4%)＞COX(7.5%)＞HSV(6.6%)＞ADV、EDV(3.8%)＞CT(2.8%)排序。三组间，B19V、CMV、RV、HSV、TOX结果的差异具有显著性(P＝0.000～P＝0.031)，其余病原体之间的差异无显著性(P＝0.121～P＝0.724)。结论病原体感染是导致不良妊娠的重要原因之一，其中B19V、CMV、TOX、RV、HSV可能是主要病原体，其余病原体对导致不良妊娠也有一定的影响。