Antibody-mediated targeting of doxorubicin-containing immunoliposomes to human gastric cancer

摘要

Doxorubicin （DXR） was entrapped in liposomes coated with themonoclonal antibody,MGb2,against human gastric cancer.Negative-stain electronmicroscopy showed the diameter of liposomes was 105.6±21.1nm.The drugentrapment efficiency was 47.6±8.1%.There were about 5667 drug moleculesentrapped and 48 antibody molecules incorporated per liposome.It was confirmedby ELISA and binding assay that the immunoliposomes could bind selectively andcarry their contents to human gastric cancer cell line,BGC-823.Theimmunoliposomes showed specific cytotoxicity to BGC-823,2.3-fold more effectivethan free DXR and 56.7-fold more effective than non-specific liposomes,but only1/25 of toxicity to normal human embryonic lung cell line,SL7,as comparedwith free DXR.This study indicates that the immunoliposomes could deliverDXR selectively to the target cells and might be useful in targeting chemotherapyof human gastric cancer.