Stereological study of quantitative relationships between morphological and functional changes of the lungs in early phase of blast injury in rats

摘要

The morphological and functional changes of the lungs in the early phase of blast injurywere analyzed and related quantitatively in rats with microcomputer-assisted morphometry.It wasfound that（1）significant or partial correlation exists between stereological and functional indices,（2）changes of the endothelium and epithelium rather than the interstitium contribute to the increase ofharmonic and arithmetic thickness(Tht,Tat)of the air-blood barrier,and（3）the regression equa-tions are as follows:（a）PaO2=3.56+1.62DL（O2）,r=0.678,P=0.032:（b）PaO2=2.99+3.49DL（O2）-0.0005SA+0.003Sc-38.2Vc-0.257Tap-0.028Dt（O2）,ry=0.9999,P=0.008;（c）PaCO2=7.69+0.0001SA-4.35Tat+19.7Tht-46.2Thp-4.4Taen-5.87Taep,ry=0.9999,P=0.014;（d）pH=3.068+0.0005SA-1.79Tat+12.2Tht-50.8Thp-2.52Taint+0.69DL（O2）,ry=0.9999,P=0.10.The significance and application of stereology in theestablishment of the quantitative relationship between morphological and functional indices are dis-cussed.Mathematical models to relate morphometric parameters to functional changes are possible.