Effects of Novel recombination human tumor necrosis factor on tumor cell growth and NF-kB activity

摘要

Objective: To investigate the relationship among the TNFRp55 and TNFRp75 expression in tumor cell lines in vitro, the suppression of nrhTNF on the tumor cell lines growth and the changing of NF-κB activity in the tumor cell lines. Methods:The expressions of TNFRs are studied by immunohistochemistry. The inhibition of nrhTNF to tumor cells was investigate by crystal violet assay. The changing of activity of NF-κB was evaluated by using immunohistochemistry and Western blot methods. Results: In tumor cell lines, positive rates of expression of TNFRp55 and TNFRp75 were 99% and 98. 8% respectively. There was no significant difference among various types of tumor cells (P＞0. 05). nrhTNF could inhibit the growth of SW480,SGC7901, 8910, SMMC7721 and BEL-7402 cell lines in which NF-κB translocated into cytonuclei slightly.However, nrhTNF has no effects on A549, PLA801, Hela, Eca109 and GRC-1 cell lines in which NF-κB translocated into cytonuclei obviously. Conclusion: The low degree of activating of NF-κB makes tumor cells sensitive to nrhTNF, however, the high degree of activating of NF-κB makes tumor cells insensitive to nrhTNF.