Effects of cholesterol liposomes on cytoskeleton and proliferation of rabbit sphincter of Oddi cells in culture

摘要

Abstract Objective:To discuss the relationship between hypercholesterolemic disease and the functional and structural changes of Sphincter of Oddi(SO) by the study of effect of Cholesterol Liposome(CL) on struc-tural and quantitative changes of SO cells .Methods:Rabbit SO was isolated for primary cell culture and sub-culture.After subcultured with different concentration of CL culture medium for 20h, the structural and quantitative changes of SO cells were analyzed and detected by MTT-test, flow cytometer(FCM), electronic microscope and electrophoresis technique respectively.Results:CL contributed a prominent stimulus to SO cells proliferation at middle concentration(1.0mg/ml) and induced apoptosis of SO cells.Swelled mitochondria and dilated endoplasmic reticulum as well as disjoined and diminished microfilaments were found in SO cells by electronic microscopy.The content of SO cells actin decreased with the increment of cholesterol concentra-tion.There was a significant difference of actin content between CL groups and control group(P<0.05).Conclusion:CL may change SO cell membrane's function, organelle's structure and especially the quantity and configuration of microfilaments, at the same time, CL at different concentration can induce changes of SO cells cycle and lead to different changes in the number of SO cells.