儿童复发性腮腺炎患者与正常儿童腮腺液微生物群落多样性的差异

摘要

目的 通过检测儿童复发性腮腺炎患者与正常儿童腮腺液的微生物群落分布的变化情况,分析二者之间微生物群落多样性的差异.方法 通过使用Illumina Hiseq 2500 PE250高通量测序平台对34例儿童复发性腮腺炎患者和16例正常儿童腮腺液标本中微生物群落的16SrRNA进行多样性分析,并对两组样本数据进行统计比较.结果 与正常儿童腮腺液对比,儿童复发性腮腺炎患者腮腺液Alpha多样性指标中样品文库的覆盖率(goods_coverage)增高(P＜ 0.05),Chaol、菌群丰富度指数(abundance coverage estimator, ACE)、Shannon指数降低(P＜0.05 ) ,simpSOn指数差异无统计学意义.结论 儿童复发性腮腺炎患者腮腺液微生态环境中微生物群落的多样性与正常儿童腮腺液差异有统计学意义.%Objective To investigate the difference in the richness and diversity of the parotid microflora between juvenile recurrent parotitis (JRP) and the normal children. Methods The parotid saliva specimens were obtained from JRP and normal children (34 specimens from JRP, 16 specimens from normal children)to analyze the diversity of the 16SrRNA gene sequence of of the microorganism using Illumina Hiseq 2500 PE250 high throughput sequencing platform. Results Compared with normal children, JRP had higher goods_coverage(P＜ 0.05), lower Chaol, abundance coverage estimator (ACE), Shannon (P＜ 0.05) and lower Simpson (P＞ 0.05). Conclusion Significant changes in the richness and diversity of parotid microflora between JRP and the normal children.