Partial Sequence Analysis of the Genome of Human Herpesvirus 7 YY5 Isolated from Saliva Samples

摘要

Objective To isolate and identify Nanjing local strains of Human Herpesvirus 7 (HH-V-7), and to analyze their partia l genome characteristic. Methods The saliva specimens were collected from 2 healthy adults and 5 children with kidney disease. After treatment with antibiotics and filtering. they were inoculated on to the phytohemagglutin stimulated umbilical cord blood mononuclear cells ( CBMCs). When the infected cells presented the typical ballooning and polykaryotic cytopathic effects (CPE), we identified them by transvnission electron microscopy and polymerase chain reaction.PCR product was also sequenced. Results Four strains were isolated from the seven saliva specimens. The 186-base-pair fragment of the isolated strain YY5 PCR products was sequenced, which encoded part of the HHV-7 U10 gene. The DNA sequence revealed an identity of 57. 5% and 36.0%, respectively with HHV-6 and human cytomegalovirus ( HCMV). At the amino acid level, the similarity of 51.6% was found between HHV-7 and HHV-6, and that of 25.8% between HHV-7and HCMV. Conclusion The isolated viruses were HHV-7, and 186 bp fragments revealed an identity with HHV-7 RK and Jl of 100%.