UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

摘要

MicroRNAs (miRNAs) are 21 to 24 nucleotide,non-coding RNA molecules that post-transcriptionally regulate the expression of target genes.Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism.In this study,we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression.Real-time RT-PCR,annexin V/fluorescein isothiocyanate staining,Western blotting and anti-miRNA oligonucleotide transfection were employed in this study.We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells.Furthermore,miR-141 expression increased apoptosis,while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB.UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells.Therefore,miR-141 is a potential gene therapy target for UVB-induced photodamage.