Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout (Lep△I14/△I14) rats

摘要

Leptin deficiency is principally linked to metabolic disorders.Leptin knockout (Lep△I14/△I14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders.We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue,liver and hypothalamus in Lep△I14△I14 and wild-type (WT) rats.We found 1,651 differentially expressed (enriched) genes in white adipose tissue,916 in the liver,and 306 in the hypothalamus in the Lep△I14/△I14 rats compared to WT.Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories,including fatty acid metabolism,molecular transducers and cellular processes.The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes.These data will contribute to a greater understanding of different metabolic disorders,such as obesity and diabetes.