Objective:It has been reported that the intravenous anesthetic propofol(PPF)has anti-inflammatory effects in vitro and in patients.The purpose of this study was to investigate whether PPF has anti-inflammatory effects in lipopolysaccharide(LPS)-induced septic shock by inhibiting the induction of pro-inflammatory cytokinesinterleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)and high mobility group box 1(HMGB1)in rats.Methods:Thirty six male Wistar rats were randomly assigned to one of three groups(control group,PPF+LPS group and LPS group;n=12 per group).Control group rats received a 0.9%NaCl solution(NS)by the tail vein.The PPF+ LPS group rats received PPF(10 mg/kg bolus,followed by infusion at 10 mg/(kg·h)through a femoral vein catheter)1 h before LPS(7.5 mg/kg)administration via the tail vein.The LPS group rats received injection of LPS(7.5 mg/kg)via the tail vein.Hemodynamic effects were recorded as well as mortality rates,and plasma cytokine concentrations(TNF-α,IL-6,HMGB1)were measured for the 24-h observation period.Results:The mean arterial pressure and heart rate of the PPF+LPS group were more stable than those of the LPS group.The mortality at 24 h after the administration of the LPS injection was much higher in the LPS group(58.3%)compared to the PPF+ LPS group(25.0%).Plasma concentrations of cytokines(IL-6 and TNF-α)and HMGB1 were significantly reduced in the PPF+LPS group compared to the LPS group(P<0.05).Conclusion:Pretreatment with PPF reduced the mortality rate of rats and attenuated the pro-inflammatory cytokine responses in an endotoxin shock model through an anti-inflammatory action inhibiting induction of HMGB1.
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