探讨氩氦靶向冻融兔肝后局部组织、超微结构变化及修复过程中的特征,为临床上肝脏肿瘤氩氦靶向治疗提供实验依据.氩氦刀置于兔肝一叶表面,冻融后在不同时间点取肝组织,在光、电镜下观察肝脏的组织与超微病理变化.冻融后第1～2d,局部冻融的肝组织从中心向外明显地分为中央区,核碎片聚积带、出血带、交界线.第4d核碎片带大多消失,近交界线上成纤维细胞增生并出现纤细的胶原纤维束.第8d交界线变成结缔组织增生带并完全包围呈溶解模糊状的冻融区,增生带外的肝组织开始增生.第16 d冻融区内的残余肝组织减少,周围的结缔组织增生带进一步增宽并不断地长入坏死区,带中出现了许多组织细胞及多核巨细胞.第30 d,底层的结缔组织带与表层的贴近形成一个“铁饼状”疤痕覆盖在新生的肝组织表面,其中的肝残余坏死成分消失.疤痕下增生的肝组织几乎取代原损伤区.全部动物中未见冻融区肝组织复活的现象.经氩氦刀冻融均破坏了冻融区内的肝组织,随时间延长经历一个溶解并逐渐被吞噬消化的过程.损伤周围逐渐形成结缔组织增生带包裹损伤区,增生带周围的肝组织逐渐增生,最后充填修复大部分损伤区.%To explore the histological and ultrastructural effects and renovating feature by Cryocare? on rabbit hepatic tissue with different freezing time and furnish an experimental basis for the clinical treatment of hepatic tumor by cryosurgery. A probe was set on the surface of rabbit lever. One group was frozen for 5 min, another 10 min . Then the hepatic tissues were taken respectively at different time points. They were observed on light and electron microscopes. The hepatic tissue was obviously divided into central area, nuclear debris accumulated band, hemorrhagic band and boundary layer the first and second day after freezing. The nuclear debris band appeared discontinuous the fourth day. There appeared proliferation band of fibroblast this phase and this band increased and produced many polynuclear giant cells gradually at the eighth and sixteenth day. At the thirtieth day the damaged area was nearly dissolved and absorbed and filled up with the new proliferated hepatocytes and only a thin layer of connective tissue on the surface in which the damaged hepatic tissue disappeared. No recovery of hepatocyte was found in the damaged area of all livers within experimental groups. The hepatic tissues in freezing areas are completely destroyed after freezing 5,10 min and will be wrapped gradually by new born connective tissue , and finally the damaged area disappears the bottom connective tissue band merges with that of the surface to form a plate - like scar tissue covering the surface of the new bom liver tissues .
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