人肠内菌对藏药余甘子鞣质部位代谢的研究

摘要

Objective To study in vitro the effect of human intestinal bacteria on the transformation of to-tal tannins extracted from emblic leafflower ( Yuganzi, Phyllanthus emblica L. ) , a usually used Tibetan herbal medicine. Methods Based on the earlier stage work of the extraction of total tannins from Phyl-lanthus emblica L. , activated human gut microbiota was anaerobically incubated in the culture medium GAM containing the total tannins at 37℃ for 72 hours. After centrifugation and protein precipitation, the characteristics of the metabolites were determined by using HPLC/MSn and the contents of each compo-nent determined were measured by using HPLC-UV, on which the time-content curve was drawn. Results Total 13 components were determined, including four metabolites ( pyrogallic acid, methyl-de-rivative of gallic, urolithin B, and urolithin A) , four prototype components ( gallic acid, corilagin, gal-loyl glucose, and digalloyl glucose) and five components to be determined. The accumulated contents of gallic acid and ellagic acid were the highest at Hour 12 , 10-fold higher than that of the beginning; the accumulated content of corilagin was the highest at Hour 6 with twice higher of the beginning. Conclusion After the total tannins from Phyllanthus emblica L. were transformed by human gut microbi-ota in vitro, certain bioactive prototype components remained and antitumor components were found.%目的：研究人肠内菌对藏药余甘子鞣质部位的代谢。方法采用人肠内菌与余甘子鞣质部位在厌氧条件下,37℃共温孵培养的方法进行代谢,代谢样品通过离心、甲醇沉淀蛋白后,运用HPLC-MS n法对代谢物进行定性分析,运用HPLC-UV法对样品进行定量分析,并绘制时间含量曲线。结果余甘子鞣质部位在人肠内菌作用下用HPLC-MSn检测出13个成分,其中4个为代谢成分焦性没食子酸、甲基没食子酸、尿石素B和尿石素A；4个为原型成分没食子酸、柯里拉京、galloyl glucose、digalloyl glucose和5个待指认成分。样品中没食子酸、柯里拉京、鞣花酸3个指标性成分的含量随人肠内菌代谢时间延长呈先增加后减少的趋势。结论余甘子鞣质部位可被人肠内菌代谢,代谢后其原型活性成分仍有保留,并且有新生成的抗癌活性成分出现。