[目的]比较不同粒径露水草(Cyanotis arachnoidea C.B.Clarke)对所含β-蜕皮激素释放的影响.[方法]采用GRACE ODS C18色谱柱(250 mm ×4.6 mm,5μm),流动相甲醇:水=40∶60( V/V,％)洗脱.柱温26℃,流速1.0 ml/min,检测波长248nm.[结果]相同来源的露水草粉体(粒径10、30和50μm)β-蜕皮激素含量检测值和普通细粉(粒径180 μm)相比,粉体中蜕皮激素激素的检测值平均高1倍多.[结论]露水草粉体中的蜕皮激素更容易溶于甲醇,其提取率明显高于普通细粉.表明露水草经过超微粉碎后,细胞破壁率到得明显提高,更有利于有效成分的溶出.%[Objective] The aim was lo investigate the influence of different size of ground Cyanotis arachnoidea C. B. Clarke particles on the release of p-ecdysone. [ Method] The content of β-eedysone extracted from Cyanotis arachnoidea powders of different sizes was detected through HPLC, under the chromatographic conditions: GRACE ODS C18 column (250 mm x 4. 6 mm, 5 μm) , mobile phase methanol: water = 40:60 ( V/V,% ) , column temperature 26℃, flow rate 1.0 ml/min and detection wavelength 248 nm. [Result] The extracted β-ecdysone from ultra-fine Cyanotis arachnoidea powder (particle size 10, 30 and 20 μm) was as more than two fold as common powder (particle size 180 μm). [ Conclusion] The β-ecdysone in ultra-fine powder of Cyanotis arachnoidea was more easily soluble in methanol. The extraction rate of p-ecdysone was significantly higher than that of common powder. The result showed that the technology of ultra-fine pulverization markedly improved the dissolution of the β-ecdysone for increasing the cell breakage rate, and then the active ingredients could be extracted more easily.
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