人参及其混伪品的紫外谱线组法鉴别研究

摘要

[Objective] To establish a method for distinguishing RADIX GINSENG from its counterfeits including RADIX PLATYCODI,RADIX ISATTDIS and RADIX GLEHNIAE by ultraviolet absorption spectra lines group (UASLG). [ Method] The 4 kinds of medicines were extracted with five different solvents of water,ethanol,petroleum ether,ethyl acetate and chloroform. Then,their absorbance in UV and one-order derivative spectra was measured. [ Result] There were obvious differences regarding peak position and absorbance in ultraviolet absorption spectra among RADIX GINSENG and RADIX PLATYCODI,RADIX ISATIDIS and RADIX GLEHNIAE. [ Conclusion]The method was simple and accurate,and could be used to distinguish RADIX GINSENG from its counterfeits.%[目的]建立鉴别人参及其混伪品桔梗、板蓝根和北沙参的紫外谱线组鉴别方法.[方法]采用水、乙醇、石油醚、乙酸乙酯和氯仿对4种药材分别进行提取,然后进行紫外检测及其一阶导数光谱检测.[结果]正品人参与桔梗、板蓝根和北沙参的紫外吸收光谱的峰位置和吸光度有明显的差异.[结论]该方法简便、准确、灵敏,能够很好的区别人参及其混伪品.