[目的]评价柳树对重金属Pb的忍耐能力及应用于植物修复的潜力.[方法]采用溶液培养研究了不同浓度Pb胁迫对苏柳172和垂柳2种柳树的根系形态、根系活力以及叶片保护酶活性和渗透调节物质的影响.[结果]Pb处理显著抑制2种柳树的根系伸长,垂柳根系表面积、根体积、根平均直径先增后降,苏柳172根系表面积、根体积、根平均直径均受到Pb的显著抑制.2种柳树的根系活力均下降:超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和脯氨酸(Pro)含量均先增加后下降;可溶性蛋白含量、叶绿紊含量在一定程度内均降低,只是变化的幅度和进程不同.[结论]为柳树应用于重金属修复具有重要的参考价值.%[ Objective ] The purpose of the research was to evaluate the potential of willow ( Salix spp. ) applying in phytoremediation of lead polluted environment. [ Method] Two willow species,Salix jiangsuensis CL‘ J172’ ,and Salix babylonica were selected and the root morphology and physical characteristic under the lead stress in solution culture were studied. [ Result ] The results showed that root length was significantly decreased under lead stress. Root surface area, root volume, and average diameter of root in Salix babylonica increased under the moderately lead stress. Root surface area,root volume,and average diameter of root in Salix jiangsuensis CL‘ J172’ were decreased significantly by lead. SOD activity, POD activity and Pro content increased under the moderately lead stress, while decline under extremely lead stress. Leaf soluble protein and chlorophyll content decrease under lead stress,though the trends differ for different species. [ Conclusion]This study had important references value to the mediation of heavy metal by willow.
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