高效液相色谱法测定新疆开心果中烟酸的含量

摘要

[Objective] To determine nicotine acid content in pistachio of Xinjiang Uygur Autonomous Region. [Method] High performance liquid chromatography was adopted to determine soluble vitamin nicotine acid in pistachio of Xinjiang. In the experiment, the column was Symmetry Shield RP18(4. 6 mm×250 mm×5 μm) using 10 mmol/L KH2PO4 as mobile phase, and it was detected by UV scanning at a wavelength of 266 nm. [ Result] The result showed that the content of nicotinic acid in pistachio of Xinjiang Uygur Autonomous Region is rich (113. 1 mg/kg), which also has high nutritional value. Consume pistachio in moderation will effectively supply people' s demand of nicotinic acid. [Conclusion] The method is simple, accurate, which will provide scientific basis for further development and application.%[目的]研究测定新疆开心果中烟酸的含量.[方法]采用高效液相色谱法测定新疆开心果中水溶性维生素烟酸的含量,试验采用Symmetry Shield RP18(4.6 mm×250 mm×5 μm)为分离柱,以10 mmol/L磷酸二氢钾为流动相,采用紫外检测器在波长266 nm下进行检测.[结果]试验得出,新疆开心果中烟酸的含量为113.1 mg/kg,表明新疆开心果中含有比较丰富的烟酸,具有较高的营养价值,适量食用开心果可有效补充人体所需的烟酸.[结论]该方法操作简单,结果准确,为新疆开心果的进一步开发应用研究提供了一定的科学依据.