双碱配合提取啤酒厂剩余污泥蛋白质的研究

摘要

[Objective] The paper aimed to improve the extraction rate of protein from brewery excess sludge. [ Method] Hydrolysis temperature, hydrolysis time,NaOH or Ca(OH)2 dosing ratio were selected as influence factors respectively,and the extraction rate of protein as evaluation value for the orthogonal experiment. Dual-alkali hydrolyzing method was used to optimize the sludge hydrolysis technology conditions based on these factors. [Result] The optimum conditions were as follows:NaOH dosing ratio(ratio of NaOH to the total dry sludge mass) 8% ,Ca(OH)2 dosing ratio 15% ,hydrolysis temperature 80 ℃ ,hydrolysis time 7 h. Under these conditions,pH value of the reaction system was 13 and extraction rate of protein could reach 60.03%. [Conclusion] The research provided a scientific basis for resource utilization of brewery excess sludge.%[目的]提高啤酒厂剩余污泥中蛋白质的提取率.[方法]分别选取水解温度、水解时间、NaOH或Ca(OH)2投加率为影响因素,以蛋白质提取率为评价指标,进行正交试验,并以此为依据采用双碱法优化污泥水解的工艺条件.[结果]双碱配合水解污泥提取蛋白质的最佳条件为NaOH投加率(占干污泥的质量分数)8％,Ca(OH)2投加率15％,水解温度80℃,水解时间7h,此时反应体系pH为13.在最优条件下,蛋白质提取率可达60.03％.[结论]该研究可为啤酒厂污泥的资源化利用提供科学依据.