厚朴叶水提物抗腹泻效果研究

摘要

[目的]为合理利用厚朴叶资源,研究不同剂量厚朴叶水提物对小鼠蓖麻油诱导腹泻的影响.[方法]以蒸馏水为阴性对照、盐酸洛哌丁胺为阳性对照,研究厚朴叶水提物低、中、高剂量组处理对蓖麻油诱导腹泻小鼠(5组× 6只)的腹泻率、起效时间、稀便次数、稀便级数和腹泻指数的影响.[结果]厚朴叶水提物低剂量组和阳性对照组均具有显著降低腹泻率的作用;随着厚朴叶水提物剂量的增加,对小鼠腹泻的稀便次数有抑制作用;厚朴叶水提物也能显著降低小鼠的稀便率,且与其剂量高低有关;厚朴叶水提物能显著降低小鼠的平均稀便等级和腹泻指数.但总的来说,厚朴叶水提物的效果不及盐酸洛哌丁胺,且对蓖麻油所致小鼠的起效时间无影响.[结论]厚朴叶水提物对蓖麻油所致的小鼠腹泻具有显著的抑制作用,且存在一定的量效关系.%[Objective]The research aimed to study the effects of different does of aqueous extraction from Magnolia officinals leaves (AEMOL) on rats diarrhea caused by castor oil, fully utilizing this resource.[Method]Using distilled water as the negative control, loperamide hydrochloride as the positive control,the effects of three different does(low, middle and high) of AEMOL on loose stools rate, onset time, numbers of loose, loose stools series and diarrhea index of the loose rats (5 groups × 6) were studied.[Result]The group of low does AEMOL and the positive control group showed significantly inhibition ratio to diarrhoea. With the does increase, it showed inhibitory effect to the number of loose;besides, AEMOL could also decrease the loose stools rate (the specific effects depend on its does);meanwhile, AEMOL could also significantly reduce the average level of loose stools and diarrhea index.But in general the effect of AEMOL was lower than hydrochloric acid, and it showed no effect on the onset time of mice induced by castor oil.[Conclusion]The AEMOL has a significant inhibitory effect on the rat diarrhea caused by castor oil with dose-effect relationship.