The purpose of this paper was to investigate the effects of iron and vitamin A on performance and serum antioxidant indices in laying hens.A 3×2 completely random experiment was designed.A total of four hundred and thirty-two Hyline Brown laying hens were randomly divided into 6 treatments.Iron supplementation levels were 0,30 and 60 mg/kg,and vitamin A supplementation levels were 4000 and 8000 IU/kg.It is found that the supplementation of dietary iron significantly increased average egg weight,the activities of Cu/Zn-superoxide dismulase,glutathione peroxidase and ceruloplasmin in serum,and the concentration of hydrogen peroxide and hydroxyl radical inhibition in serum（P0.05）,and significantly increased total antioxidant capacity in serum at 31-34 weeks（P0.05）.The supplementation of dietary vitamin A significantly increased the activity of ceruloplasmin in serum（P0.05）.Interaction between iron and vitamin A significantly affected the activities of glutathione peroxidase and ceruloplasmin in serum（P0.05）.It is concluded that the supplementation of iron in diet did not improve the production performance of laying hens,but promoted the production of free radicals,and increased the activity of antioxidase.%为探讨铁和维生素A及其互作效应对产蛋鸡生产性能和血清抗氧化指标的影响,采用3×2完全随机试验设计,将432只海兰褐产蛋鸡随机分为6个处理组。日粮铁的添加量为0、30、60mg/kg,维生素A的添加量为4000、8000IU/kg。结果表明：（1）日粮铁的添加水平显著提高平均蛋重和血清铜锌超氧化物歧化酶、谷胱甘肽过氧化物酶和铜蓝蛋白活力及过氧化氢含量和抑制羟自由基能力（P〈0.05）,显著提高31~34周龄总抗氧化能力（P〈0.05）。（2）日粮维生素A的添加水平显著提高了血清铜蓝蛋白活力（P〈0.05）。（3）铁和维生素A互作效应对血清谷胱甘肽过氧化物酶和铜蓝蛋白活力的影响均显著（P〈0.05）。结果说明常规日粮中添加铁没有明显改善产蛋鸡的生产性能,但促进了体内自由基的生成,同时提高了抗氧化酶的活力。
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