褐化是植物组织培养中一种常见的问题,直接影响组织培养能否成功。为了控制植物组织培养中的褐化现象,选取3个玉米自交系丹黄25、P138、H21的幼胚为外植体,着重探讨胚龄,2,4-D、甘露醇、L-脯氨酸和AgNO3浓度对玉米幼胚愈伤组织褐化的影响。结果表明,选取授粉11天的‘丹黄25’幼胚、13天的P138和H21幼胚接种到诱导培养基中,待长出愈伤后转移到添加2mg/L2,4-D、10g/L甘露醇、10mg/LL-脯氨酸、10mg/L硝酸银的继代培养基上,愈伤组织褐化率显著降低,表明培养基的种类及添加物对愈伤组织的生长有重要影响。%Material browning was a universal phenomenon in plant-tissue culture, and it play a vital role in plant-tissue culture. To control the browing of plant-tissue culture, the immature embryos of three maize inbred lines （Danhuang 25, P138, H21） were used in the experiment. Effect of embryo age, 2,4-D concentration, mannitol concentration, L-proline and silver nitrate concentration on the browning of maize calli were studied. The results showed that the embryos selected from ‘Danhuang 25’ （11-day after self-pollination）, P138 and H21 （13-day after self-pollination） were inoculated into induction medium and then their callus browning could be significantly inhibited by addition of 2 mg/L 2, 4-D, 10 g/L mannitol, 10 mg/L L-proline, 10 mg/L silver nitrate in subculture medium, indicated that the type of medium and additived have a major impact on callus growth.
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