Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs

摘要

cqvip:Cellular retinoic acid-binding protein 1(CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression(LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel(IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profi les in prenatal skeletal muscle(33, 65 and 90 days post coitus, dpc) from Landrace(lean-type)(described as L33, L65 and L90) and Tongcheng pigs(obese-type)(described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms(SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281(G>A) and g. 2992(G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY(Duroc×(Landrace×Yorkshire)) pig breeds.