cqvip:The lower seed setting is one of the major hindrances which face grain yield in rice.One of the main reasons to cause low spikelet fertility(seed setting)is male sterility or pollen abortion.Notably,pollen abortion has been frequently observed in advanced progenies of rice.In the present study,149 BC_2F_6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212(indica)and Nipponbare(japonica)and used for primary gene mapping.Three QTLs,qSS-1,qSS-7 and qSS-9 at chromosomes 1,7 and 9,respectively,were found to be associated with seed setting.The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1,qSS-7 and qSS-9 loci were mapped to an interval of 188,701 and 3741 kb,respectively.The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC_2F_7 population of 1 849 individuals.There are 16possible putative genes in this 93.5 kb region.Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal.These data showed that low seed setting rate relative to qSS-1may be caused by abnormal pollen grains.These results will be useful for cloning,functional analysis of the target gene governing spikelet fertility(seed setting)and understanding the genetic bases of pollen sterility.
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