GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean

摘要

cqvip:The NAC(NAM, ATAF1/2 and CUC2) transcription factor family plays a key role in plant development and responses to abiotic stress. GmN AC15(Glyma15 g40510.1), a member of the NAC transcription factor family in soybean, was functionally characterized, especially with regard to its role in salt tolerance. In the present study, q RT-PCR(quantitative reverse transcription PCR) analysis indicated that Gm NAC15 was induced by salt, drought, low temperature stress, and ABA treatment in roots and leaves. Gm NAC15 overexpression in soybean(Glycine max) hairy roots enhanced salt tolerance. Transgenic hairy roots improved the survival of wild leaves; however, overexpression of Gm NAC15 in hairy root couldn't influnce the expression level of Gm NAC15 in leaf. Gm NAC15 regulates the expression levels of genes responsive to salt stress. Altogether, these results provide experimental evidence of the positive effect of Gm NAC15 on salt tolerance in soybean and the potential application of genetic manipulation to enhance the salt tolerance of important crops.