鹰嘴豆芽素A对多种肿瘤细胞株抑制效果的研究

摘要

目的：研究鹰嘴豆芽素A对多种肿瘤细胞株抑制率的影响。方法 MTT法测定鹰嘴豆芽素A对肿瘤细胞的抑制作用，根据测得的OD值计算肿瘤细胞抑制率，计算出 IC50（半数抑制浓度）值。结果鹰嘴豆芽素A对人类结肠癌细胞（HT－29），人前列腺癌细胞（PC－3），人肺癌细胞（NCI－H157），人宫颈癌（Hela），人乳腺癌细胞（MCF－7）的IC 50分别为28．37±2．35μM，19．16±3．08μM，9．26±1．98μM，35．65±2．07μM和37．38±2．65μM。相对其他细胞株而言，对人肺癌细胞NCI－H157的抑制作用最为显著。鹰嘴豆芽素A对正常细胞非洲绿猴肾细胞（ Vero）的IC50为134．06±2．62μM，表明其对正常的细胞毒性较小。结论鹰嘴豆芽素A对被检测的多种肿瘤细胞株均有一定的抑制效果。%Objective This study was designed to explore the inhibitory effect of Biochanin A towards several tumor cell lines.Methods MTT assay was used to detect the inhibitory effect of Biochanin A towards several tumor cell lines. The IC50 values were obtained according to OD values. Results Our results showedthatBiochaninAsignifi-cantlyinhibitedseveraltumorcelllines growth,includinghuman colon cancer cells (HT-29,IC50:28.37 ±2.35 μM),hu-manprostatecancer cells ( PC -3, IC 50: 19.16 ± 3.08 μM),humanlungcancer cells ( NCI -H157, IC 50:9.26 ±1.98 μM) , humancervical cancer cells ( Hela, IC50:35.65 ±2.07 μM) andhumanbreast cancer cells (MCF -7, IC50: 37.38 ±2.65 μM), suggesting that NCI -H157 cell line is the most sensitive.However, the IC50 of African green monkey kidney ( Vero ) cellswas 134.06 ± 2.62 μM.Conclusions Biochanin A can inhibit significantly the growth of several tumor cell lines.