Acute and sub-acute toxicity study ofClerodendrum inerme, Jasminum mesnyi Hance andCallistemon citrinus

摘要

Objective:To study acute and sub-acute toxicity study ofClerodendrum inerme(C. inerme), Jasminum mesnyi(J. mesnyi)Hance andCallistemon citrinus(C. citrinus). Methods:The acute toxicity test was conducted inSwiss albino mice.The extracts ofC. inerme,J. mesnyiHance and C. citrinus was administered in single dose of0.5,1.0,2.0,3.0,4.0 and5.0 g/kg and observed for behavioral changes and mortality, if any.In sub-acute toxicity study,Wistar rats of either sex were administered1/5th of the maximum tolerated dose,p.o. for4 weeks.Rats were observed weekly for any change in their body weight, food and water intake during28 d of the treatment.At the end of28 d, blood samples of the rats were collected for hematological and biochemical study. Results:In acute toxicity study, all four extracts of three plants were found to be well tolerated up to the dose of2000 mg/kg.These produced neither mortality nor any change in the behavior in mice.In sub-acute toxicity study, all four extracts of three plants at theLD50 dose level did not produce any significant alteration in hematological and biochemical parameters in rats. Conclusions:The results demonstrated that there is a wide margin of safety for the therapeutic use of each of the four extracts of three plants.The findings also corroborated the traditional use of these extracts.