人 Mcl －1基因启动子区结合蛋白的生物信息学分析

摘要

目的：对人 Mcl －1基因启动子区结合蛋白进行生物信息学分析。方法：从 GeneBank 数据库中获取人Mcl －1基因5′调控区，使用在线软件 JASPAR 对该序列可能存在的转录因子结合位点进行预测。结果：Relative profile score threshold 在85％、90％、95％和100％时，该序列存在1700、1147、368和32个可能的转录因子结合位点，包含 Ar-id3a，Atoh1，ETV6，Gata1，GATA3，KLF5，LMX1A，LMX1B，MZF1，Prrx2，SPI1，TBX4，USF1，YY1，ZEB1等。结论：人 Mcl －1基因5′调控区存在多种转录因子的结合位点，这些转录因子大多与肿瘤发生，红细胞生成及神经发育等有关。%Objective To analysis of transcription factor binding site in the promoter of human Mcl -1 gene.Method The 5′regulatory region of Mcl -1 gene was obtained from GeneBank database.The transcriptional binding sites of Mcl -1 gene were predicted by online software JASPAR.Results 1700,1147,368 and 32 potential transcription factors binding sites were predicted in the 5′regulatory region of Mcl -1 gene when the relative profile score higher than 85%,90%,95% and 100%,including Ar-id3a,Atoh1,ETV6,Gata1,GATA3,KLF5,LMX1A,LMX1B,MZF1,Prrx2,SPI1,TBX4,USF1,YY1,ZEB1 and et al.Conclusion There are many transcription factor binding sites of human Mcl -1 gene 5′regulatory region,which are associated with regulation of tumor occurrence,erythrocytopoiesis and neurodevelopment.