目的 探讨日光灯长期照射对SD大鼠视网膜钟基因隐色素2(Cry2)表达的影响.方法 健康SD大鼠30只,随机分成2组,每组15只.实验组接受人工光源循环照射,对照组接受自然光线的照射,观察时间3个月.采用免疫组织化学法和实时定量-PCR检测Cry2蛋白和Cry2mRNA在视网膜组织的表达.结果 两组视网膜节细胞及部分内核层细胞均有Cry2蛋白的阳性表达.实验组Cry2蛋白和Cry2 mRNA表达均较对照组减少(P＜0.05).结论 日光灯长期照射可引起视网膜钟基因Cry2的表达降低,提示光照对视网膜生物钟可能产生影响.%Objective To investigate the effect of artificial light on the expression of cryptochrome 2(Cry2) in the retina of rats. Methods Thirty SD rats were equally randomized into 2 groups of A(exposed to artificial light for 3 months) and B(exposed to natural light as the control). The expressions of Cry2 protein and Cry2 mRNA were detected by immunohistochemistry and Q-PCR technique. Results The positive expression of Cry2 protein was found in most of the ganglion cell layers and some of inner nuclear layers in both groups. The expressions of of Cry2 protein and Cry2 mRNA were lower in group A than those in group B(P<0. 05). Conclusion Artificial light exposure for a long time can cause less expression of Cry2 in the retina of rats,suggesting that artificial light can affect the circadian clock system in the retina of rats.
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