山绿茶总黄酮对前列腺增生大鼠细胞增殖的影响

摘要

Objective To observe the effect of Hainan holly leaf total flavonoids(HHLTF) on the expressions of proliferating cell nuclear antigen (PCNA) and Ki-67 in rats with prostatic hyperplasia(PH). Methods Forty-eight male SD rats were equally randomized into six groups of P (PH model+gastric gavage with normal saline), Q(PH model+gastric gavage with Qianliekang 570 mg · kg-1 · d-1), HI, H2, H3 (PH model + gastric gavage with HHLTF 80,40, 20 mg · kg1 · d-1, respectively) and CXgastric gavage with normal saline as blank control). The PH model was made by subcutaneous injection of testosterone propionate after surgical castration and treatment lasted for 5 weeks, after which the index of prostate gland (PI) was calculated and the expressions of PCNA and Ki-67 in prostate tissues were detected by immunohistochemistry. Results PI and the expressions of PCNA and Ki-67 in prostate tissues were significantly lower in groups of HI and H2 than those in group P(P<0. 01). Conclusion HHLTF can inhibit proliferation of the prostate gland, which may be through inhibiting the expressions of PCNA and Ki-67.%目的 观察前列腺增生大鼠前列腺组织增殖细胞核抗原(PCNA)和Ki-67的表达及山绿茶总黄酮(HHLTF)对其影响.方法 48只SD大鼠随机均分为前列腺增生模型(P)组、前列康治疗(Q)组、HHLTF高(H1)、中(H2)、低(H3)剂量治疗组和对照(C)组.除C组外各组大鼠去势后注射丙酸睾酮制作前列腺增生模型,用前列康(570mg·kg1·d-1)或HHLTF 80、40、20 mg·kg1 ·d-l灌胃,连续5周；P、C组以生理盐水灌胃.计算大鼠前列腺指数,免疫组化法测定PCNA和Ki-67的表达.结果 H1、H2组的前列腺指数及PCNA、Ki-67表达均较P组显著降低(P.＜0.01).结论 HHLTF具有抑制前列腺增生的作用,其机制可能是通过抑制PCNA和Ki-67蛋白的表达而实现.