杂交捕获法和PCR-RDB法检测妇女HPV感染的比较

摘要

Objective To compare hybrid capture-Ⅱ(HC2)to polymerase chain reaction(PCR)-reverse dot-blot(RDB)for detecting HPV infection in women.Methods HC2 method and PCR-RDB were applied to detecting human papillomaviral infection in female genital duct,and the test results were analyzed.Results Concordant results were found in 81.14%(400/493)samples(Kappa=0.63;95%CI,0.57-0.69).The high risk type HPV positive rate detected by PCR-RDB were 1.60%(3/187),29.85%(20/67),69.88%(58/83),86.52%(77/89)and 91.04%(61/67),respectively,when the HC2 RLU/CO of samples were＜1.00,1.00-9.99,10.00-99.99,100.00-999.99 and≥1000.00.Statistical differences were found among the five groups(χ2=289.3,P＜0.01).Cross reactions were found between HC2 high risk probe and HPV53,66,11,cp8304 and other genotypes.Conclusion There is good concordance between HC2 and PCR-RDB,though cross reaction exists in HC2.Relatively speaking,there are more influential factors for PCR-RDB.%目的 探讨HC2法和PCR-RDB法检测妇女HPV感染的异同点.方法 利用HC2法和PCR-RDB法检测妇女生殖道HPV感染状况,然后进行比较.结果 81.14%(400/493)的样本PCR-RDB法和HC2法检测结果一致,两法一致性检验Kappa值为0.63(95%CI,0.57～0.69).在HC2法RLU/CO＜1.00、1.00～9.99、10.00～99.99、100.00～999.99和≥1000.00的样本中,PCR-RDB法13种高危型HPV的检出率依次为1.60%(3/187)、29.85%(20/67)、69.88%(58/83)、86.52%(77/89)和91.04%(61/67),五组间比较,差异有统计学意义(χ2=289.3,P＜0.01).HC2法高危型HPV试剂可与HPV53、66、11,cp8304等基因型发生交叉反应.结论 HC2法和PCR-RDB法具有较好的一致性,HC2法存在交叉反应现象,PCR-RDB法难以标准化.