肿瘤标志物DJ-1、NSE、CYFRA21-1和CEA联合检测在肺癌诊断中的应用价值

摘要

Objective To study the serum level of DJ-1 ,neuronspecific enolase(NSE) ,cytokeratin 19 fragment 21-KCYFRA21-1) , carcino-embryonic antigen(CEA) in cases and their value in diagnosis of lung cancer. Methods Serum DJ-1 (with enzyme-linked immunosorbent assay) ,NSE,CYFRA21-1 ,CEA( with electrochemiluminescence)level were detected in 60 patients with lung cancer, 60 patients with pulmonary infection and 60 healthy controls . Results 4 serum tumor markers in patients with lung cancer were significant higher than those in patients with pulmonary infection and healthy controls (P<0. 01); the sensitivity, specificity and Youden index of DJ-1,NSE,CYFRA21-1,CEA combined detection were 85%,78. 3% and 0. 63 in lung cancer. Conclusion DJ-1, NSE,CYFRA21-1 and CEA levels have significance in detection of lung cancer, which provide evidence for diagnosis of lung cancer. Combined detection with 4 tumor markers can improve sensitivity and specificity in diagnosis of lung cancer.%目的 探讨肿瘤标志物D J-1蛋白(DJ-1)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段21-1 (CYFRA21-1)及癌胚抗原(CEA)在肺癌患者血清中的水平以及在肺癌诊断中的价值.方法 采用酶联免疫吸附法(ELISA)检测血清DJ-1蛋白,采用电化学发光法(ECLIA)检测血清NSE、CYFRA21-1和CEA水平,比较分析肺癌组、肺部良性疾病组和健康对照组的各60例病例.结果 肺癌组的4种血清肿瘤标志物水平均高于肺部良性疾病组和健康对照组,差异有统计学意义(P＜0.01)；肺癌病理类型中鳞癌中有较高的DJ-1和CYFRA21-1水平,腺癌中有较高的CEA水平,而小细胞癌中有较高的NSE水平；以上4种肿瘤标志物联合检测肺癌的诊断价值最高,灵敏度为85％,特异度为78.3％,约登指数达到0.63.结论 DJ-1、NSE、CYFRA21-1及CEA联合检测对肺癌的诊断有一定的价值,可以作为高危人群初步筛查的指标.