高尔基体糖蛋白73、磷脂酰肌醇蛋白聚糖3、甲胎蛋白异质体百分比联合检测在原发性肝癌诊断中的应用

摘要

Objective To study the application of the combined detection of Golgi glycoprotein 73(GP-73),phosphatidylinositol proteoglycan 3(GPC3 )and percentage of AFP heteroplasmon(AFP-L3%)in the diagnosis of primary hepatocellular carcinoma (PHC).Methods The concentrations of GP-73,GPC3 and AFP-L3 were detected by enzyme-linked immunosorbent assay(ELISA) in 154 patients with PHC(PHC group),78 patients with cirrhosis(cirrhosis group)and 56 healthy subjects(control group).Then the detection results were statistically analyzed.Results The levels of GP-73,GPC3 and AFP-L3% in the PHC group were signifi-cantly higher than those in the liver cirrhosis group and the control group(P <0.05).The positive rates of GP-73,GPC3 and AFP-L3% in the PHC group were 66.2%,72.1% and 53.2% respectively.The positive rate in the combined detection of these three in-dices could reach 97.9%,which was higher than the sensitivity and accuracy in any single index detection and the combination de-tection.In the PHC group,the comparison between different levels of GP-73 and AFP-L3% with the AFP levels showed the statis-tically significant difference(P <0.05 ).Conclusion The combination detection of GP-73,GPC3 and AFP-L3% can improve the sensitivity and accuracy for diagnosing PHC and has reference significance in the differential diagnosis of early PHC.%目的：探讨高尔基体糖蛋白73（GP-73）、磷脂酰肌醇蛋白聚糖3（GPC3）、甲胎蛋白异质体百分比（AFP-L3％）联合检测在原发性肝癌诊断中的应用。方法选择肝癌患者154例（肝癌组），肝硬化患者78例（肝硬化组），健康对照者56例（对照组），用酶联免疫法分别测出 GP-73、GPC3、AFP-L3的浓度。然后分别按照检测项目统计分析。结果肝癌组中 GP-73、GPC3、AFP-L3％的水平明显高于肝硬化组和对照组（P ＜0．05）；肝癌组中 GP-73、GPC3、AFP-L3％的阳性率分别为66．2％、72．1％、53．2％。三项联合检测的阳性率可达到97．9％。高于任何单项和联合检测的敏感度和准确度。肝癌组中 GP-73、AFP-L3％在 AFP不同水平内比较，差异有统计学意义（P ＜0．05）。结论 GP-73、GPC3、AFP-L3％三者联合检测提高原发性肝癌诊断的敏感度和准确度，对鉴别诊断早期肝癌有参考意义。